AZDye555 NT Labeling Kit
Fluorescent DNA labeling kit based on nick translation technology for preparing labeled DNA probes for fluorescence-based nucleic acid detection and hybridization applications.
Product Information
| Catalogue Number | 0220-PP-305S-AZ555 |
| Product Size | 10 Reactions |
| Labeling Technology | Nick Translation (NT) |
| Fluorescent Label | AZDye555 |
| Research Area | Molecular Biology |
| Dry Ice | No |
| Hazardous | No |
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product Description
AZDye555 NT Labeling Kit contains the reagents required for DNA labeling by nick translation, with the exception of the DNA template and materials needed for probe purification. It provides an efficient and convenient enzymatic labeling method for preparing fluorescently labeled DNA probes.
The AZDye555 NT labeling mix contains specially optimized dUTP-XX-AZDye555, which is incorporated into DNA during nick translation using DNA Polymerase I. The fluorophore combines good stability and quantum yield with an efficient dye-dUTP incorporation rate.
The resulting labeled double-stranded DNA fragments are suitable for fluorescence-based applications, including fluorescence in situ hybridization (FISH), microarray gene expression profiling, and other nucleic acid hybridization assays.
Principle of Nick Translation Labeling
Nick translation is an enzymatic DNA labeling method that uses the coordinated activities of DNase I and DNA Polymerase I to introduce fluorescently labeled nucleotides into double-stranded DNA.
DNase I introduces randomly distributed nicks into double-stranded DNA at low enzyme concentrations.
The 5′→3′ exonuclease activity of DNA Polymerase I removes nucleotides ahead of the nick while its polymerase activity synthesizes a new complementary DNA strand from the available 3′-OH terminus.
In the presence of dye-labeled dUTP, DNA Polymerase I incorporates AZDye555-labeled dUTP in place of dTTP during DNA synthesis.
The balanced polymerase and nuclease activities generate fluorescently labeled double-stranded DNA fragments suitable for downstream hybridization applications.
Key Features & Benefits
- Efficient fluorescent labeling: Incorporates AZDye555-labeled dUTP into DNA through enzymatic nick translation.
- Ready-to-use labeling reagents: Contains the reagents required for the labeling reaction, excluding the DNA template and probe purification materials.
- Fluorescence performance: Combines fluorophore stability and quantum yield with a high incorporation rate.
- Double-stranded DNA labeling: Produces fluorescently labeled DNA fragments.
- Convenient workflow: Uses the coordinated activities of DNase I and DNA Polymerase I.
- Broad research applications: Suitable for FISH, microarray gene expression profiling, and nucleic acid hybridization assays.
Research Applications
- Fluorescence in situ hybridization (FISH)
- Microarray gene expression profiling
- Fluorescent DNA probe preparation
- Nucleic acid hybridization assays
- Fluorescence-based molecular biology research
Technical Specifications
| Product Name | AZDye555 NT Labeling Kit |
| Alternative Name | Yellow fluorescent DNA labeling by nick translation |
| Catalog Number | 0220-PP-305S-AZ555 |
| Pack Size | 10 Reactions |
| Labeling Method | Nick translation |
| Fluorescent Nucleotide | dUTP-XX-AZDye555 |
| Enzymatic Components | DNA Polymerase I and DNase I activities |
| Template | Not included |
| Probe Purification Materials | Not included |
| UNSPSC | 41106300 |
| HS Code | 38221900 |
| eClass Number | 32160502 |
| eClass Product Group | PCR-Reagenz (Labor) |
| Autoclavability | — |
Storage & Handling
- Storage Temperature: −20°C
- Storage Conditions: Store protected from light.
- Freeze-Thaw: Avoid repeated freeze-thaw cycles.
- Shipping Conditions: Shipped on gel packs.
- Shelf Life: 12 months.
Product Identifiers
| Synonym | Yellow fluorescent DNA labeling by nick translation |
| UNSPSC Description | Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products |
SMILES
C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]\([C@H]\(O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC[N+]1=C(/C=C/C=C/2\C(c3c(N2CCCS(=O)(=O)O)ccc(c3)S(=O)(=O)O)(C)C)C(c2c1ccc(c2)S(=O)(=O)[O-])(C)CCCCS(=O)(=O)O